pbluescript ii ks plasmid (Agilent technologies)
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pbluescript ii ks plasmid
Pbluescript Ii Ks Plasmid, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescript+ii+ks+plasmid/pm38294527-49-18-22
Average 90 stars, based on 1 article reviews
Pbluescript Ii Ks Plasmid, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescript+ii+ks+plasmid/pm38294527-49-18-22
Average 90 stars, based on 1 article reviews
pbluescript ii ks plasmid - by Bioz Stars,
2026-09
90/100 stars
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Sequencing:Article Title: Development of Lentiviral Vectors Simultaneously Expressing Multiple siRNAs Against CCR5 , vif and tat/rev Genes for an HIV-1 Gene Therapy Approach Article Snippet: .. To obtain the shCCR5, the shvif and the scrambled sequence, two complementary DNA oligonucleotides flanked by Mlu I and Cla I sites were synthesized, annealed, and inserted at the EcoR V site of the Article Title: Mirk/Dyrk1B controls ventral spinal cord development via Shh pathway Article Snippet: For Sonic Hedgehog riboprobes, we cloned a cDNA fragment of 498 bp corresponding to the 5’-coding region of mSHH by applying PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491) in pCMV6-Entry plasmid, containing mShh coding region Myc-DDK-tagged (Mouse Sonic hedgehog NM_009170, ORIGENE, MR227201), and primers mSHHFOR: 5’-CCCAAGCTTGCCAGCGGCAGATAT-3’ and mSHHREV: 5’- CGGGAATTCGTCCTTCACCAGCTTG-3’. .. The PCR product of 498 bp, was then subcloned into the Hind III and EcoRI restriction sites of Article Title: Mirk/Dyrk1B controls ventral spinal cord development via Shh pathway. Article Snippet: For Sonic Hedgehog riboprobes, we cloned a cDNA fragment of 498 bp corresponding to the 5’-coding region of mSHH by applying PCR with Q5 HighFidelity DNA Polymerase (New England Biolabs, M0491) in pCMV6-Entry plasmid, containing mShh coding region Myc-DDK-tagged (Mouse Sonic hedgehog NM_009170, ORIGENE, MR227201), and primers mSHHFOR: 5’-CCC AAG CTT GCC AGC GGC AGA TAT -3’ and mSHHREV: 5’- CGG GAA TTC GTC CTT CAC CAG CTT G-3’. .. The PCR product of 498 bp, was then subcloned into the Hind III and EcoRI restriction sites of Synthesized:Article Title: Development of Lentiviral Vectors Simultaneously Expressing Multiple siRNAs Against CCR5 , vif and tat/rev Genes for an HIV-1 Gene Therapy Approach Article Snippet: .. To obtain the shCCR5, the shvif and the scrambled sequence, two complementary DNA oligonucleotides flanked by Mlu I and Cla I sites were synthesized, annealed, and inserted at the EcoR V site of the Plasmid Preparation:Article Title: Development of Lentiviral Vectors Simultaneously Expressing Multiple siRNAs Against CCR5 , vif and tat/rev Genes for an HIV-1 Gene Therapy Approach Article Snippet: .. To obtain the shCCR5, the shvif and the scrambled sequence, two complementary DNA oligonucleotides flanked by Mlu I and Cla I sites were synthesized, annealed, and inserted at the EcoR V site of the Article Title: Combined magnetic tweezers and micro-mirror total internal reflection fluorescence microscope for single molecule manipulation and visualization Article Snippet: 2.1.6 DNA tethering Anti-digoxigenin, polyclonal (11333089001, Roche) 1X Phosphate buffered saline (PBS) Trizma base NaCl Products from DNA ligation reaction Bovine serum albumin (BSA) Tween-20 Magnetic beads (Dynabeads MyOne Streptavidin T1; 35601, Invitrogen) Wash buffer (WB): 1x PBS, 0.1% w/v BSA, 0.01% v/v Tween-20 Bead wash buffer (BWB): 10 mM Tris (pH 7.5), 1 M NaCl 2.1.7 TIRF calibration Biotinylated fluorescent nanodiamonds ( e.g. Adamas Nanodiamonds) 2.1.8 General supplies 200 μl thin-wall PCR tubes 1.5 ml Eppendorf tubes .. pET28b plasmid (EMD4Biosciences) Article Title: Expression of Xanthophyllomyces dendrorhous cytochrome-P450 hydroxylase and reductase in Mucor circinelloides. Article Snippet: Carotenoids are natural pigments that act as powerful antioxidants and have various beneficial effects on human and animal health.. Mucor circinelloides (Mucoromycotina) is a carotenoid producing zygomycetes fungus, which accumulates b-carotene as the main carotenoid but also able to produce the hydroxylated derivatives of b-carotene (i.e. zeaxanthin and b-cryptoxanthin) in low amount.. These xanthophylls, together with the ketolated derivatives of b-carotene (such as canthaxanthin, echinenone and astaxanthin) have better antioxidant activity than b-carotene. Article Title: Mirk/Dyrk1B controls ventral spinal cord development via Shh pathway Article Snippet: For Sonic Hedgehog riboprobes, we cloned a cDNA fragment of 498 bp corresponding to the 5’-coding region of mSHH by applying PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491) in pCMV6-Entry plasmid, containing mShh coding region Myc-DDK-tagged (Mouse Sonic hedgehog NM_009170, ORIGENE, MR227201), and primers mSHHFOR: 5’-CCCAAGCTTGCCAGCGGCAGATAT-3’ and mSHHREV: 5’- CGGGAATTCGTCCTTCACCAGCTTG-3’. .. The PCR product of 498 bp, was then subcloned into the Hind III and EcoRI restriction sites of Article Title: Mirk/Dyrk1B controls ventral spinal cord development via Shh pathway Article Snippet: .. For in situ hybridization (ISH) experiments, we used a cDNA fragment of 253 bp corresponding to the 3’-coding region of cDyrk1B cDNA, a cDNA fragment of 396 bp corresponding to the 3’-coding region of mDyrk1B cDNA, both of them subcloned into the Hind III and EcoRI restriction sites of Article Title: Beta-actin and RPS21 promoters and uses thereof Article Snippet: .. These were subsequently cloned into Article Title: Analysis of the 9p21.3 sequence associated with coronary artery disease reveals a tendency for duplication in a CAD patient Article Snippet: .. In parallel, the primers B635 and B629 ( ) were used to PCR amplify the Article Title: Mirk/Dyrk1B controls ventral spinal cord development via Shh pathway. Article Snippet: For Sonic Hedgehog riboprobes, we cloned a cDNA fragment of 498 bp corresponding to the 5’-coding region of mSHH by applying PCR with Q5 HighFidelity DNA Polymerase (New England Biolabs, M0491) in pCMV6-Entry plasmid, containing mShh coding region Myc-DDK-tagged (Mouse Sonic hedgehog NM_009170, ORIGENE, MR227201), and primers mSHHFOR: 5’-CCC AAG CTT GCC AGC GGC AGA TAT -3’ and mSHHREV: 5’- CGG GAA TTC GTC CTT CAC CAG CTT G-3’. .. The PCR product of 498 bp, was then subcloned into the Hind III and EcoRI restriction sites of Expressing:Article Title: Expression of Xanthophyllomyces dendrorhous cytochrome-P450 hydroxylase and reductase in Mucor circinelloides. Article Snippet: Carotenoids are natural pigments that act as powerful antioxidants and have various beneficial effects on human and animal health.. Mucor circinelloides (Mucoromycotina) is a carotenoid producing zygomycetes fungus, which accumulates b-carotene as the main carotenoid but also able to produce the hydroxylated derivatives of b-carotene (i.e. zeaxanthin and b-cryptoxanthin) in low amount.. These xanthophylls, together with the ketolated derivatives of b-carotene (such as canthaxanthin, echinenone and astaxanthin) have better antioxidant activity than b-carotene. Transformation Assay:Article Title: Expression of Xanthophyllomyces dendrorhous cytochrome-P450 hydroxylase and reductase in Mucor circinelloides. Article Snippet: Carotenoids are natural pigments that act as powerful antioxidants and have various beneficial effects on human and animal health.. Mucor circinelloides (Mucoromycotina) is a carotenoid producing zygomycetes fungus, which accumulates b-carotene as the main carotenoid but also able to produce the hydroxylated derivatives of b-carotene (i.e. zeaxanthin and b-cryptoxanthin) in low amount.. These xanthophylls, together with the ketolated derivatives of b-carotene (such as canthaxanthin, echinenone and astaxanthin) have better antioxidant activity than b-carotene. Amplification:Article Title: Expression of Xanthophyllomyces dendrorhous cytochrome-P450 hydroxylase and reductase in Mucor circinelloides. Article Snippet: Carotenoids are natural pigments that act as powerful antioxidants and have various beneficial effects on human and animal health.. Mucor circinelloides (Mucoromycotina) is a carotenoid producing zygomycetes fungus, which accumulates b-carotene as the main carotenoid but also able to produce the hydroxylated derivatives of b-carotene (i.e. zeaxanthin and b-cryptoxanthin) in low amount.. These xanthophylls, together with the ketolated derivatives of b-carotene (such as canthaxanthin, echinenone and astaxanthin) have better antioxidant activity than b-carotene. Clone Assay:Article Title: Expression of Xanthophyllomyces dendrorhous cytochrome-P450 hydroxylase and reductase in Mucor circinelloides. Article Snippet: Carotenoids are natural pigments that act as powerful antioxidants and have various beneficial effects on human and animal health.. Mucor circinelloides (Mucoromycotina) is a carotenoid producing zygomycetes fungus, which accumulates b-carotene as the main carotenoid but also able to produce the hydroxylated derivatives of b-carotene (i.e. zeaxanthin and b-cryptoxanthin) in low amount.. These xanthophylls, together with the ketolated derivatives of b-carotene (such as canthaxanthin, echinenone and astaxanthin) have better antioxidant activity than b-carotene. Article Title: Beta-actin and RPS21 promoters and uses thereof Article Snippet: .. These were subsequently cloned into Polymerase Chain Reaction:Article Title: Mirk/Dyrk1B controls ventral spinal cord development via Shh pathway Article Snippet: For Sonic Hedgehog riboprobes, we cloned a cDNA fragment of 498 bp corresponding to the 5’-coding region of mSHH by applying PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491) in pCMV6-Entry plasmid, containing mShh coding region Myc-DDK-tagged (Mouse Sonic hedgehog NM_009170, ORIGENE, MR227201), and primers mSHHFOR: 5’-CCCAAGCTTGCCAGCGGCAGATAT-3’ and mSHHREV: 5’- CGGGAATTCGTCCTTCACCAGCTTG-3’. .. The PCR product of 498 bp, was then subcloned into the Hind III and EcoRI restriction sites of Article Title: Analysis of the 9p21.3 sequence associated with coronary artery disease reveals a tendency for duplication in a CAD patient Article Snippet: .. In parallel, the primers B635 and B629 ( ) were used to PCR amplify the Article Title: Mirk/Dyrk1B controls ventral spinal cord development via Shh pathway. Article Snippet: For Sonic Hedgehog riboprobes, we cloned a cDNA fragment of 498 bp corresponding to the 5’-coding region of mSHH by applying PCR with Q5 HighFidelity DNA Polymerase (New England Biolabs, M0491) in pCMV6-Entry plasmid, containing mShh coding region Myc-DDK-tagged (Mouse Sonic hedgehog NM_009170, ORIGENE, MR227201), and primers mSHHFOR: 5’-CCC AAG CTT GCC AGC GGC AGA TAT -3’ and mSHHREV: 5’- CGG GAA TTC GTC CTT CAC CAG CTT G-3’. .. The PCR product of 498 bp, was then subcloned into the Hind III and EcoRI restriction sites of In Situ Hybridization:Article Title: Mirk/Dyrk1B controls ventral spinal cord development via Shh pathway Article Snippet: .. For in situ hybridization (ISH) experiments, we used a cDNA fragment of 253 bp corresponding to the 3’-coding region of cDyrk1B cDNA, a cDNA fragment of 396 bp corresponding to the 3’-coding region of mDyrk1B cDNA, both of them subcloned into the Hind III and EcoRI restriction sites of |
